最新刊期
卷 3 ， 期 1 ， 2024
- 摘要：Sperm-like stem cells are a new type of haploid embryonic stem cells derived from mouse androgenetic blastocysts. They contain only paternal genetic material， with the X chromosome as the sex chromosome， and are capable of long-term self-renewal， proliferation and induced differentiation in vitro. The combination of sperm-like stem cells and CRISPR/Cas9 technology enables the injection of gene-edited sperm-like stem cells into oocytes to obtain genotypically determined semi-cloned mice efficiently and stably. Sperm-like stem cell-mediated genetic modification has a wide range of application prospects including efficient establishment of animal models， rapid simulation of complex diseases， and mining new diagnostic and therapeutic methods. It also allows for targeted genetic screening at the individual level in animal models and promotes large-scale genome tagging projects.关键词：sperm-like stem cell;semi-cloning technology;mouse disease model;CRISPR/Cas9;genome editing;genome tagging project
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：Collagen is an important component of extracellular matrix in human body， with the special "triple helix" structure. Collagen is both structural protein and functional protein. And it is also an important biomedical material. Currently， collagen is mainly obtained on the market through animal extraction， which cannot avoid the risk of immunogenicity caused by species differences and the risk of animal pathogens infection. Meanwhile， low abundance collagen cannot be obtained through animal tissue extraction. Recombinant humanized collagen， especially through functional region strategies， not only avoids immunogenicity， but also guarantees good collagen biocompatibility and activity. This paper focuses mainly on the research development of the new material of type A recombinant humanized collagen type Ⅲ， and analyzes its possible innovative application scenarios. Firstly， we obtained the core functional region of human type Ⅲ collagen with a special 164.88° triple helix structure by high-throughput screening. Subsequently， we performed 16 linear repeats of the core functional region to obtain A-type recombinant humanized collagen type Ⅲ without any exogenous non-human collagen sequence in its amino acid sequence. A-type recombinant humanized collagen type Ⅲ has a triple helix structure and does not enter the cell when interacting with the cell. A-type recombinant humanized collagen type Ⅲ effectively exerts repair and regeneration efficacy by improving the extracellular matrix， which is expected to play an important role in the fields of skin damage repair， gynaecology， cardiovascular， orthopaedics， stomatology and so on. Now， A-type recombinant humanized collagen type Ⅲ occupies a major position in the market in the field of injectable recombinant collagen， which is a breakthrough of 40 years of only-animal collagen injections.关键词：synthetic organisms;biomedical materials;human collagen type Ⅲ;recombinant humanized collagen;Type A;triple helix structure
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：As an important chemical intermediate， long-chain dicarboxylic acid is widely used in the manufacture of engineering plastics， high temperature lubricants， surfactant， cosmetics， etc. The global demand for long-chain dicarboxylic acid is increasing in recent years. This article， taking sebacic acid for example， summarizes and evaluates the current chemical and biological synthesis methods of sebacic acid， and also briefly introduces its industrialization progress.关键词：sebacic acid;long-chain dicarboxylic acid;chemical synthesis method;biocatalytic method;industrialization
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：The emergence of gene therapy in precision medicine has made a profound impact on healthcare and diagnostics. As leading gene delivery tools in gene therapy， adeno-associated virus （AAV） gene vectors are clinically approved carriers， ensuring safety， low immunogenicity， diverse serotypes， tissue specificity， and long-term effectiveness in vivo. However， gene over-expression delivered by AAV may lead to toxicity or side effects. Consequently， new AAV vectors engineered through synthetic biology are on the rise. Synthetic biology can control the function of carriers at different stages of cellular transduction and gene expression， thus precisely controlling target gene expression. This article focuses on the design principles and methods of gene circuits， advancements in AAV gene therapy research， and progress in synthetic biology within the context of AAV gene therapy. It provides insights into the prospective clinical applications and the challenges of AAV gene therapy driven by synthetic biology. Synthetic biology-driven AAV gene therapy has exhibited substantial potential in providing precise and personalized treatment solutions， with the promise of expanding the therapeutic scope and reducing costs. However， it also faces key challenges， including immune responses， targeting specificity， production costs， and stringent ethical and regulatory considerations.关键词：synthetic biology;adeno-associated virus;gene therapy;gene circuits
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：As a resource-based economic province， it is of great significance to achieve carbon peaking and carbon neutrality goals in Shanxi province. Synthetic biology significantly reduces carbon emissions through the use of synthetic biotechnologies， meeting the requirements of green and low-carbon development. It also represents one of the key industrial chains that Shanxi focuses on. In October 2020， Shanxi Comprehensive Reform Demonstration Zone introduced Cathay Biotech in response to the national "dual carbon" strategic objectives， initiating the world’s first one million tons bio-based new material project， and commenced the construction of "Shanxi Synthetic Biology Industry Ecological Park"， thereby creating a complete industrial cluster design. To promote the development of the synthetic biology industry in Shanxi， the province pioneered talent cultivation in synthetic biology. In February 2021， school of synthetic biology in Shanxi university was inaugurated by Lou Yangsheng， the then secretary of Shanxi provincial party committee. The school received approval from the Ministry of Education in 2022 to set up an undergraduate program in synthetic biology； in 2023， it was authorized to establish an interdisciplinary master’s and doctoral degree program in the field， culminating in a comprehensive integrated training system from undergraduate to doctoral levels. The approved application for the establishment of the synthetic biology industry college aims to create an institution with systemic innovation and distinctive features， and is committed to high-quality talents cultivation and technological innovation in the field of synthetic biology， and thus promoting Shanxi province’s economic transformation and leapfrog development. This paper summarizes from a global perspective the current state and the trends of synthetic biology industry and its academic discipline construction.关键词：peak carbon dioxide emissions;carbon neutrality;synthetic biology;synthetic biology industry;school of synthetic biology
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：As a group of aquatic photosynthetic autotrophic microorganisms， microalgae play an important role in sustaining the earth's ecosystems， which depends on the efficient photosynthetic carbon sequestration efficiency. Studies have been committed to exploring the efficient carbon sequestration mechanisms and the functions of various bioactive compounds of microalgae since last century. The natural high-efficiency carbon sequestration ability， the rich pigment types and the high lipid content of microalgae give them a unique advantage in photosynthetic carbon fixation， high-value pigments， and biofuels. Traditionally， excellent strains have often been isolated and screened to improve the yield of production and efficiency of carbon fixation. However， the low biomass of microalgae prevents its advantages from perfectly integrating with economic industries. Synthetic biology provides a new avenue for modifying and optimizing microalgae. This review displays the applications and future directions of synthetic biology in carbon neutrality， high-value pigments and biofuels of microalgae.关键词：microalgae;synthetic biology;carbon neutrality;high-value pigments;biofuel
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：Synthetic biology uses the concept of "engineering design" to design and even re-synthesize organisms in order to create artificial organisms and products with specific and unnatural functions. Countries around the world are accelerating the application of synthetic biotechnology in agriculture to cope with global climate change， population growth， environmental pollution and resource scarcity， and to promote the realization of the "dual carbon" goal. There are lots of insects in nature， among them， the Orthoptera agricultural pests Locusta migratoria and Gryllus bimaculatus are easy to cultivate and rich in protein， fat， amino acids， minerals and vitamins. They are important protein source in agricultural production， and has become the new resource insect. Insect production consumes less energy. To produce the same amount of protein， the insect production consumes far less water， feed and greenhouse gas emissions than traditional livestock and poultry farming. It has been researched and developed as a future food in the European Union countries and Japan. Gene editing technology represented by CRISPR/Cas9 is an important technology in the field of synthetic biology. It can achieve targeted gene knockout， insertion， and modification at the genomic level to regulate gene expression and species traits. The promotion and application in resource insects will promote the development of high-quality resource insect populations and strains， and help the rapid development of synthetic biology agriculture.关键词：synthetic biology;agriculture;gene editing;Locusta migratoria;Gryllus bimaculatus
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：RISPR-Cas genome editing tool has gained popularity due to its simplicity， efficiency， and universality in creating targeted mutations in animals and plants. By fusing additional structural domains， e. g. activation domains， inhibition domains， deaminases， reverse transcriptases， etc. onto Cas proteins， the CRISPR-Cas system can be endowed with novel functions. Among them， base editors can precisely replace bases at specific sites in DNA or RNA without causing double-strand breaks， thereby effectively inducing genetic mutations. Since the invention of base editors， researchers have been working on modifying Cas proteins， deaminases， and construction strategies to address key characteristics of base editors， including the types of base substitutions， editing window， editing specificity， editing range， sequence preferences， and editing purity. This article presents an overview of various base editors developed in plants， evaluates comprehensively their strengths and limitations， and discusses the potential applications in agriculture.关键词：CRISPR-Cas;deaminases;base editors;plant
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：Lignin is an abundant source of aromatics in nature. However， the sustainable utilization of lignin is continuously confined by its degradation and transformation due to the structural complexity and intrinsic heterogeneity. The emerging synthetic biology provides technical support for lignin valorization. This review introduces briefly several strategies of lignin depolymerization and discusses comprehensively the application of synthetic biology in converting lignin to value-added chemicals. In addition， the challenges of research and application in lignin biotransformation is prospected.关键词：Lignin;synthetic biology;biotransformation;value-added chemicals
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15 - 摘要：Artificial life is an interdisciplinary study on life itself and life process， and it is a diversified research field， including dry artificial life and wet artificial life. Dry artificial life is divided into soft artificial life which uses digital structure to create simulation or display life behavior， and hard artificial life which uses hardware to produce realistic system. The field of wet artificial life is derived from molecular biology， the synthesis of living systems through biochemical substances， i.e.， synthetic biology. A common theme in both fields is to construct living systems from non-living parts and to build detailed life models. This paper will focus on the two fields of artificial life， and sort out the source between them from the historical perspective， so as to better explore and understand the mystery of life.关键词：synthetic biology;hard artificial life;bio-robot
- 2
- |
- 0
- |
- 0
发布时间：2024-08-15
- Tel：0351-7652230 Email：est@sxu.edu.cn
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0